This lab runs the HIV-1 and HIV-2 RNA and DNA PCRs using the Cobas ampliprep/ Taqman platform plus the recently acquired Abbot m2000sp and Abbott m2000rt platform. PBMC preparations are also undertaken here.
For the last 8 years, this laboratory has support VL testing for the majority of HIV care and treatment programs in Uganda. The laboratory further support the roll out of the national EID program, providing DNA PCR testing for more than 18 organizations and facilities across the country
The main analyzer is a Roche COBAS Integra which is fully automated for performing clinical chemistry plus a Roche Elecys. test menus include albumin, alkaline phosphatase, ALT, AST, Bilirubin, total and direct, BUN, Calcium, Chloride, Carbon Dioxide, Cholesterol, HDL-C, LDL-C, creatinine, glucose, magnesium, sodium, phosphorus, potassium, total protein, protein CSF/urine, creatinine kinase, GGT, lipase, lactate, uric acid, LDH, amylase, both total and pancreatic, and Hepatitis B and C.
The analyzers in use are two Coulter-Beckman ACT5 Diffs and ACL 9000 for coagulation studies. Test menu includes CBC, malaria smears, prothrombin time, activated thromboplastin time, sickling test, ABO Group and cross matching.
This lab was established in 1998 to perform immunology research assays mainly in HIV, TB and HIV and TB co-infection. This state-of-art laboratory is well equipped with two Class II biosafety cabinets, three centrifuges, a CO2 incubator, two ELISA plate readers, five refrigerators, a vacuum pump, six -80ºC freezers, 10 liquid nitrogen tanks, three computers, and a seven color flow cytometer.
This laboratory is performing a number of immunological assays including but not limited to peripheral blood/pleural fluid mononuclear cell (PBMC) isolation and storage, flow cytometry for cell phenotypic analysis and polyfunctionality, tissue/cell cultures for cytokine production, ELISAs for detection of cytokines, Elispots for detection of cytokines produced by different cell populations in response to multiple antigens, separation of cell populations by rosettesep, and column separation technique, DNA and RNA preparation from buffy coat and Paxgene preparations and separation and storage of serum, plasma, and buffy coat, for detection of various blood components.