This study builds on research conducted in the early 2000s that examined the relationship between hormonal contraceptive use, genital tract microbiome diversity and HIV seroconversion. Archived biological samples from the original cohorts were securely stored and later retrieved in 2024. The participants vaginal swabs were processed and analyzed for genital tract microbiome shedding. Furthermore, contemporary molecular methods, including nucleic acid extraction, amplification, and sequencing, to generate HIV genotypic resistance profiles. The project leverages historical cohorts to address current research questions on HIV drug resistance using modern laboratory technologies.
Expected impact: Evidence on HIV genotypic resistance sequences derived from archived samples, comparative analyses linking historical cohort data with current resistance profiles, and characterization of genital tract microbiome
